What Is SH-SY5Y Neuroblastoma Cells? Uses & Culture

In the world of neuroscience, sh-sy5y is a key tool for scientists. Known as sh sy5y atcc CRL-2266, it helps us understand human brain diseases. This thrice-cloned subline gives us a closer look at brain functions and how diseases spread.

This line comes from a 1970 biopsy of a four-year-old’s bone tumor. It’s called united 2266 in science. It lets us study how human neurons work in a controlled setting. Learning about shsy5y is key to finding new medical solutions.

We aim to help scientists by sharing our knowledge on these tools. By studying them, we can change how we study and treat brain diseases. We’re dedicated to giving you the basics you need for your lab work.

Key Takeaways

• The SH-SY5Y line is a thrice-cloned subline derived from the parental SK-N-SH source.
• These samples are officially cataloged under the designation ATCC CRL-2266.
• The original biopsy was obtained from a four-year-old patient in 1970.
• Researchers utilize this model to study neuronal differentiation and disease mechanisms.
• This line serves as a reproducible, cost-effective tool for global neuroscience studies.

Origins and Development of SH-SY5Y Neuroblastoma Cells

The story of creating specialized cell lines is truly fascinating. It shows how important genetic stability is for research. Neuroblastoma cells are key, coming from the SK-N-SH line, known as ATCC HTB-11.

Tracing the Lineage from SK-N-SH

The sh-sy5y cell line is a special part of the SK-N-SH culture. Scientists picked certain traits to make a better model for studying the brain. This makes shsy5y cells consistent in their traits, no matter where they’re used.

This isn’t just luck. It’s the result of careful selection. By focusing on specific traits, researchers have made the culture better. This helps make sure scientific results can be trusted.

The Sequential Cloning Process

To grasp the subline meaning, we need to understand the cloning steps. Moving from the original line to sh sy 5y is a detailed process. Each step makes the culture more uniform.

The table below shows how these cells were developed. It shows how each step leads to the final, specialized line used today:

Research Applications and Scientific Utility of Neuroblastoma Cells

Scientific progress relies on the models we use in our experiments. Sy-sh5y cells are key for studying the human nervous system. They can turn into neuron-like cells, helping us find new medical solutions.

Key Areas of Neuroscience Research

The sy5y cell is used in many areas of science. We study neurodegenerative diseases like Alzheimer’s and Parkinson’s with them. These cells help us understand how these diseases work.

These cells are also vital in neurotoxicology. By testing sh sh5y cells with toxins, we learn how they affect neurons. They also help us study how the nervous system and immune system interact.

Standard Culture Protocol and Maintenance

To keep sh5y cells healthy, we follow strict lab rules. We use a mix of DMEM and Hams F12 medium with 10 percent fetal bovine serum. This mix gives the cells the nutrients they need to grow well.

To succeed in your lab, follow this sh sy5y cell culture protocol closely:

• Environment: Keep a humid chamber at 37 degrees Celsius with 5 percent carbon dioxide.
• Subcultivation: Use a 1:20 to 1:50 ratio to avoid overcrowding.
• Maintenance: Change the medium every 4 to 7 days to keep conditions stable.

By sticking to these steps, your cells will grow well. They will double every 67.3 hours. This consistency is key for reliable and meaningful results.

Conclusion

Medical science needs reliable models that act like human biology. The sy5y cell line is key for researchers studying the brain. It helps labs understand how neurological conditions progress.

Working with the sh-sy5y cell requires precision. Keeping culture practices consistent is essential. This ensures your data is strong and can be trusted by others worldwide.

We support your drive for excellence in every experiment. Our team offers the tools you need to work with sy5y cells effectively. Contact us to see how we can help your lab make a difference in patient care globally. Your hard work is shaping the future of healthcare.

References 

National Center for Biotechnology Information. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5336396/