Last Updated on November 17, 2025 by Ugurkan Demir

Knowing how to do a bone marrow aspiration procedure is key to good results. At Liv Hospital, we teach doctors the important steps and landmarks for this critical task.The bone marrow aspiration procedure is crucial. Learn the powerful steps and key landmarks instantly in this guide now.
A bone marrow aspiration takes a sample of liquid marrow, usually from the iliac crest. It’s a vital tool for checking blood-related conditions. Our team makes sure patients get the best care.
Key Takeaways
- Knowing the key landmarks for bone marrow aspiration is vital for success.
- The iliac crest is the most common place for bone marrow aspiration.
- Using the right technique keeps patients comfortable and ensures accurate results.
- Liv Hospital offers detailed guidance on bone marrow aspiration procedures.
- Getting a correct diagnosis through bone marrow aspiration is key to treatment planning.
Understanding Bone Marrow Aspiration

Bone marrow aspiration is key to diagnosing blood-related disorders. It involves taking a sample of the bone marrow’s liquid part. This is often done with a bone marrow biopsy.
Definition and Clinical Significance
Bone marrow aspiration is a medical procedure to collect bone marrow for tests. It’s important because it lets doctors check the bone marrow’s health. They can diagnose blood-related conditions and see if treatments are working.
This procedure helps us find out what’s wrong with a patient. It’s used for conditions like leukemia and anemia. The results help us create the right treatment plan.
Types of Bone Marrow Samples
There are two main types of bone marrow samples: aspirate and biopsy. The aspirate is the liquid part, used to check for cell problems. The biopsy is a solid piece of tissue, showing the marrow’s structure.
We use different methods to get these samples. They work together to fully understand the bone marrow’s state.
| Sample Type | Description | Clinical Use |
| Aspirate | Liquid portion of bone marrow | Assess cellular composition, detect abnormalities |
| Biopsy | Solid core of bone marrow tissue | Evaluate marrow architecture and cellularity |
Differences Between Aspiration and Biopsy
The main difference is the sample type and what it shows. Aspiration gives a liquid sample for cell checks. A biopsy provides a solid tissue sample for structural insights.
Both are vital and usually done together. They help fully evaluate the bone marrow. Common sites for these procedures are the posterior and anterior iliac crests.
Indications for Bone Marrow Examination

It’s key for healthcare pros to know when to do a bone marrow test. This test is a big help in finding and tracking blood and marrow diseases, like some cancers.
Diagnostic Indications
There are many reasons to do a bone marrow test. It’s used to find the cause of low blood cell counts and to spot cancers like leukemia and lymphoma. It’s also used for diseases that spread into the bone marrow.
This test is vital for figuring out why blood cell counts are off. It’s also key in finding infections and inflammation in the bone marrow.
| Diagnostic Indication | Description |
| Unexplained Cytopenias | Diagnosing the cause of low blood cell counts |
| Hematologic Malignancies | Diagnosing cancers such as leukemia and lymphoma |
| Suspected Marrow Infiltrative Diseases | Identifying diseases that infiltrate the bone marrow |
Staging and Monitoring Indications
Bone marrow tests are also used to check how far a disease has spread or if treatment is working. We use biopsies to see how much disease is in the bone marrow, like in lymphoma. This helps us see if treatment is working.
In patients with blood cancers, these tests help us see how well they’re responding to treatment.
Contraindications and Precautions
Even though bone marrow tests are very helpful, there are times when they shouldn’t be done. We have to be careful with patients who have severe bleeding problems or are on blood thinners.
We also have to be very careful to avoid infections. Making sure the patient is comfortable and safe is our top priority. We take steps to manage pain and anxiety.
Anatomical Landmarks for Bone Marrow Aspiration
Bone marrow aspiration needs precise knowledge of certain body parts. This ensures the procedure is safe and works well. The iliac crest area is key to understanding.
Posterior Iliac Crest Anatomy
The posterior iliac crest is the top choice for bone marrow aspiration. It’s important to find the posterior superior iliac spine (PSIS). This spot is easy to touch and guides the needle.
Anterior Iliac Crest Approach
The anterior iliac crest is another option. Locating the anterior superior iliac spine (ASIS) is critical. The ASIS is a key spot for this approach.
Alternative Bone Marrow Aspiration Sites
While the iliac crest is most used, other spots like the sternum or tibia might be chosen. The decision depends on the patient’s body and the doctor’s choice.
| Anatomical Landmark | Location | Clinical Significance |
| Posterior Superior Iliac Spine (PSIS) | Posterior iliac crest | Guides needle insertion for bone marrow aspiration |
| Anterior Superior Iliac Spine (ASIS) | Anterior iliac crest | Alternative landmark for bone marrow aspiration |
We usually place patients in a lateral decubitus or prone position. This makes it easier to reach the iliac crest. Knowing these spots and how to position patients is key to a successful procedure.
Patient Positioning for Bone Marrow Aspiration
Bone marrow aspiration needs precise patient positioning for safety and success. The right position is key to accessing the iliac crest comfortably and safely. This is a vital part of the procedure.
Patients are usually placed in a lateral decubitus or prone position. This helps reach the iliac crest. The choice depends on the patient’s comfort, the doctor’s preference, and clinical needs.
Lateral Decubitus Position Technique
The lateral decubitus position has the patient lying on their side. It’s often chosen because it makes accessing the posterior iliac crest easy. To set this up:
- Have the patient lie on their side with their knees bent.
- Make sure the patient’s hips are aligned for better access to the iliac crest.
- Use pillows or supports to keep the patient comfortable and stable.
Prone Position Technique
The prone position has the patient lying on their stomach. It’s useful for reaching the posterior iliac crest, mainly when the lateral decubitus position is not possible.
| Positioning Technique | Key Considerations |
| Lateral Decubitus | Patient lies on their side, knees flexed; easy access to the posterior iliac crest. |
| Prone | Patient lies on their stomach; useful for accessing the posterior iliac crest when lateral decubitus is not feasible. |
Medical experts stress that proper positioning is not just about comfort. It’s also about ensuring the procedure’s success and avoiding complications.
“The position of the patient is critical for the operator to get a good bone marrow sample.”
In summary, the right positioning for bone marrow aspiration is essential. It greatly affects the procedure’s success. By picking the best position, healthcare providers can make the procedure safe and effective.
Equipment and Materials Required
To do a bone marrow aspiration procedure well, you need the right tools. The right equipment makes sure the procedure goes smoothly. It also keeps the patient safe and comfortable.
Aspiration Needles and Selection
Choosing the right needle is key to a good bone marrow sample. Needles made for bone marrow aspiration are bigger and stronger than regular ones. We pick a needle based on the patient’s age, size, and what the procedure needs.
There are many types of needles for bone marrow aspiration. These include:
- Reusable needles with a stylet to prevent clogging
- Disposable needles to lower infection risk
- Needles with adjustable guards for precise depth
We pick a needle that fits the patient’s body and the procedure’s needs.
Additional Equipment Checklist
For a bone marrow aspiration, you need more than just a needle. You also need:
| Equipment | Purpose |
| Syringes (10-20 mL) | For taking out bone marrow |
| Local anesthetic (e.g., lidocaine) | To numb the skin and bone |
| Antiseptic solution | To clean the skin |
| Sterile drapes and gloves | To keep everything clean |
Having all your equipment ready and easy to get to is important. We also make sure everything is clean, and we have enough for any extra needs.
Pre-Procedure Protocol
A detailed pre-procedure plan is key for bone marrow aspiration. It helps reduce risks and improve results. We make sure all important steps are done before the procedure. This ensures patient safety and the success of the bone marrow aspiration.
Patient Assessment and Laboratory Testing
We start by thoroughly assessing the patient. We look at their clinical indication, primary diagnosis, and coagulation profile. Laboratory tests, like a complete blood count and coagulation studies, are vital. They help spot any bleeding risks or complications.
Laboratory tests check the patient’s overall health. They help find any issues that could affect the procedure. We also review the patient’s medical history. This helps us understand if any conditions might impact the bone marrow aspiration.
Informed Consent Process
Getting informed consent is a big part of the pre-procedure plan. We make sure patients know the risks, benefits, and alternatives of the procedure. We have a clear talk about what they can expect during and after the procedure.
Medication Considerations
We look closely at the patient’s medications. We check if any could affect the bone marrow aspiration. Anticoagulant medications are a special concern. We might need to stop or adjust them before the procedure to lower bleeding risks.
Step-by-Step Bone Aspiration Procedure
To get a successful bone marrow sample, we need to follow a detailed process. This includes picking the right spot, getting ready, and inserting the needle. This step is key to getting a sample that helps doctors diagnose and plan treatments.
Site Selection and Marking
The first thing we do is pick and mark the right spot. The back of the pelvis is often chosen because it’s easy to reach and gives good marrow. Getting the marking right is very important for the procedure’s success.
“Choosing the right spot for the aspiration is very important,” say doctors. It depends on the patient’s body and what the procedure requires. Aseptic Technique Application
After marking the spot, we use a clean technique to avoid infection. We clean the area with an antiseptic and cover it to keep it clean. Following this clean technique closely is key to keeping the patient safe.
Local Anesthesia Administration
To make the patient comfortable, we give local anesthesia at the marked spot. This is a big step toward reducing pain and worry. The type of anesthesia and how it’s given can really affect the patient’s experience.
Needle Insertion Technique
The last step is putting the needle into the bone marrow. We need to push firmly but carefully to go through the bone and into the marrow. Doing this right is important for getting a good sample without hurting the tissue around it.
By being careful and following these steps, we can make sure the bone marrow aspiration goes well. This way, we get the information needed for diagnosis while keeping the patient comfortable and safe.
Bone Marrow Aspiration Iliac Crest Technique
The iliac crest is often chosen for bone marrow aspiration because it’s easy to access. It’s a safe spot to get bone marrow samples. These samples are key to making diagnoses.
Posterior Iliac Crest Approach
The posterior iliac crest is favored for its size and easy access to the marrow. To start, the patient is placed in a specific position. This could be on their side or lying face down, depending on the situation.
Next, the anatomical landmarks are found. The posterior superior iliac spine (PSIS) is felt, and the needle spot is marked below and to the side. Local anesthesia is given to reduce pain.
Common Technical Challenges
Even though the procedure is straightforward, there are technical hurdles. One big one is needle placement. If the needle isn’t in the right spot, the sample might not be good. Knowing the anatomy well and being precise is key.
Keeping the patient comfortable is another challenge. Using enough local anesthesia and gentle pressure helps. Also, explaining the process clearly can ease their anxiety.
Knowing the posterior iliac crest method and the possible problems helps doctors. It makes bone marrow aspiration more successful and better for patients.
Specimen Collection and Processing
After the bone marrow aspiration, we focus on collecting and processing the specimen. This is key to getting accurate results. We handle the sample carefully to keep it in good condition for analysis.
Aspiration Sample Handling
Dealing with the aspiration sample needs a lot of attention. We must make sure the sample stays clean and undamaged. It’s important to place the sample gently on a clean slide or in a container ready for analysis.
To handle the sample right, we stick to a set protocol. This includes:
- Using sterile tools to handle the sample
- Keeping it away from extreme temperatures
- Processing it quickly after it’s collected
Smear Preparation Techniques
Creating smears from the bone marrow aspirate is a vital step. We make smears by spreading the aspirate on glass slides and then drying or fixing them for staining and looking under a microscope.
| Smear Preparation Technique | Description | Advantages |
| Direct Smear | Aspirate is directly spread onto a slide | Quick and simple |
| Concentrate Smear | Aspirate is concentrated before smearing | Enhances cellular detail |
| Crush Smear | Gentle crushing between slides to spread cells | Useful for cellular aggregates |
Specimen Labeling and Transport
Labeling and transporting the specimen correctly is vital. We label each sample with the patient’s information and the analysis needed.
We use special containers to protect the sample during transport. The choice of transport medium and conditions depends on the analysis needed.
Post-Procedure Care and Complications
After a bone marrow aspiration, it’s important to follow the care instructions closely. This helps avoid complications and makes recovery easier. We watch patients for any immediate issues and give them detailed home care instructions.
Immediate Post-Procedure Care
Right after the procedure, we check the patient’s vital signs and watch the site for bleeding or swelling. We also check for pain or discomfort that might need extra care.
Key aspects of immediate care include:
- Monitoring vital signs
- Observing the aspiration site
- Managing pain effectively
Potential Complications
Bone marrow aspiration is usually safe, but complications can happen. These might include infection, bleeding, or nerve damage. It’s important for patients to know about these risks and when to get medical help.
| Complication | Symptoms to Watch For | Action to Take |
| Infection | Increased redness, swelling, or pus at the aspiration site, fever | Contact your healthcare provider immediately |
| Bleeding | Excessive bleeding or bruising at the aspiration site | Apply pressure and contact your healthcare provider |
| Nerve Damage | Numbness, tingling, or persistent pain | Seek medical attention promptly |
Patient Discharge Instructions
Before leaving, we give patients clear instructions. They learn how to care for the site, manage pain, and watch for complications. We tell them to avoid hard activities and keep the site clean and dry.
Conclusion
Knowing the key steps and landmarks in bone marrow aspiration is vital for doctors. We’ve covered the main points for a successful procedure. This includes the importance of knowing the right spots and using the right technique.
By sticking to these guidelines, doctors can do bone marrow aspiration safely and well. They use important landmarks to guide them. This procedure is a key tool for diagnosing and treating patients.
We’ve talked about how bone marrow aspiration needs a deep understanding of its steps and landmarks. With the right training, doctors can get better at this. This improves care and results for patients.
FAQ
What is bone marrow aspiration?
Bone marrow aspiration is a test where a sample of bone marrow is taken. It’s usually done from the iliac crest. This helps doctors check for blood-related problems.
What are the indications for bone marrow examination?
Doctors use bone marrow tests to find the cause of low blood counts or cancer. They also use it to track how a disease is progressing and if treatments are working.
What are the anatomical landmarks for bone marrow aspiration?
The main spots for bone marrow tests are the iliac crest. There are other places too, depending on the patient’s needs and the doctor’s plan.
How is patient positioning done for bone marrow aspiration?
Patients are placed in a way that makes it easy to get to the bone marrow. They might lie on their side or on their stomach. This makes them comfortable and helps the doctor.
What equipment is required for bone marrow aspiration?
You need special needles for the test. The doctor picks the right one based on the patient. You also need gloves, drapes, and anesthetics to keep everything clean and pain-free.
What is the pre-procedure protocol for bone marrow aspiration?
Before the test, the doctor checks the patient’s health and blood work. They get the patient’s consent and make sure any medicines won’t interfere with the test.
How is a bone marrow aspiration procedure performed?
First, the doctor marks the spot. Then, they clean the area and numb it. Next, they insert the needle and collect the sample. The sample is then sent for analysis.
What are the common technical challenges during bone marrow aspiration?
Sometimes, it’s hard to get to the bone marrow. The marrow might be too dense or fibrotic. Managing pain or anxiety is also a challenge.
How is the aspiration sample handled and processed?
The sample is handled carefully to avoid contamination. Smears are made using special techniques to keep the sample alive for analysis.
What is the post-procedure care for bone marrow aspiration?
After the test, the patient gets immediate care. They are watched for any complications. The doctor also gives them instructions for a safe recovery at home.
What are the possible complications of bone marrow aspiration?
Rare complications include bleeding, infection, and nerve damage. But these are usually avoided if the procedure is done correctly.
Can bone marrow aspiration be performed on alternative sites?
Yes, other places like the sternum or tibia can be used. But the iliac crest is the most common site.
How is the success of bone marrow aspiration ensured?
Success comes from proper positioning, accurate needle placement, and careful handling of the sample. This ensures the test is done right.
References
- Masian, A., Matalon, S., & Moy, L. (2022). Bone marrow aspiration and biopsy. Radiographics, 42(1), 170-184. ps://pubmed.ncbi.nlm.nih.gov/34398308/[11 –